Polymerization behavior of Klenow fragment and Taq DNA polymerase in short primer extension reactions
نویسندگان
چکیده
منابع مشابه
Polymerization behavior of Klenow fragment and Taq DNA polymerase in short primer extension reactions.
DNA polymerases amplify DNA fragments through primer extension reactions. However, polymerization behavior of short primers in the primer extension process has not been systematically explored. In this study, we examined the minimal primer length required for primer extension, and the effect of primer length, mismatches and other conditions on DNA polymerization using a non-radioactive method. ...
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ژورنال
عنوان ژورنال: Acta Biochimica et Biophysica Sinica
سال: 2010
ISSN: 1672-9145,1745-7270
DOI: 10.1093/abbs/gmq082